Glandular Odontogenic Cyst Associated with Impacted Tooth: A Case Report

Authors

1 Dental Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

2 Oral and Maxillofacial Diseases Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Glandular odontogenic cyst (GOC) is an uncommon developmental cyst. It accounts for 0.012% to 1.3% of all the jaw cysts; its prevalence is 0.17%. It was described by Gardner et al. in 1988 as a distinct entity. GOC has a slight predilection for mandible and affect more commonly in the middle-aged patients. Radiographic and clinical features of this cyst are not pathognomonic or specific. Only the histopathological examinations allow for certain diagnosis of the cyst. The increased recurrence rate can be due to cell kinetics in the lining epithelium, multilocularity and incomplete removal of the lining following conservative treatment. In this report, we describe a case of GOC of the mandible associated with impacted tooth. The clinical diagnosis of dentigerous cyst was made, whereas histopathological examination results in a diagnosis of glandular odontogenic cyst. Based on clinical and radiological examination the diagnosis of other lesions such as radicular cyst, dentigerous cyst, and ameloblastoma may be made but histologically GOC shows certain characteristic features.

Keywords


Case Report

 

 


Glandular Odontogenic Cyst Associated with Impacted Tooth:

 A Case Report

 

Jahanshah Salehinejad1, Shadi Saghafi2, Narges Ghazi1

 

1 Dental Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

2 Oral and Maxillofacial Diseases Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran

 

Received 2 January 2013 and Accepted 23 February 2013

 


Abstract

Glandular odontogenic cyst (GOC) is an uncommon developmental cyst. It accounts for 0.012% to 1.3% of all the jaw cysts; its prevalence is 0.17%. It was described by Gardner et al. in 1988 as a distinct entity. GOC has a slight predilection for mandible and affect more commonly in the middle-aged patients. Radiographic and clinical features of this cyst are not pathognomonic or specific. Only the histopathological examinations allow for certain diagnosis of the cyst. The increased recurrence rate can be due to cell kinetics in the lining epithelium, multilocularity and incomplete removal of the lining following conservative treatment. In this report, we describe a case of GOC of the mandible associated with impacted tooth. The clinical diagnosis of dentigerous cyst was made, whereas histopathological examination results in a diagnosis of glandular odontogenic cyst. Based on clinical and radiological examination the diagnosis of other lesions such as radicular cyst, dentigerous cyst, and ameloblastoma may be made but histologically GOC shows certain characteristic features.

 

Key Words: Glandular odontogenic cyst, impacted tooth, mandible.

 

 

 

 

 

 

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Salehinejad J, Saghafi S, Ghazi N. Glandular Odontogenic Cyst Associated with Impacted Tooth: A Case Report. J Dent Mater Tech 2013; 2(3): 99-103.

Introduction

Glandular odontogenic cyst (GOC) is an uncommon developmental jaw cyst with special histopathological features and biological behavior (1,2). In 1987, Padyachee and Van Wyk (3) reported the first two cases of this cyst as a Sialo-Odontogenic Cyst.

This cyst was established as a distinct entity by Gardner et al. (4) in 1988, under the term of a glandular odontogenic cyst. In 1992, the GOC was included in the list of histologic type of odontogenic cysts by the world health organization (WHO) with the term of "Sialo-Odontogenic Cyst" or a "Glandular Odontogenic Cyst". The frequency of GOC has been reported to be 0.012% to 1.3% of all the jaw cysts and its prevalence is 0.17% (1). Clinically the most common site of occurrence is mandible, especially in the anterior region. GOC has a slight male predilection and occurs primarily in middle-aged patients (1,5). Radiographically, these cysts reveal a unilocular or multilocular radiolucency with well-defined borders (6).

On the base of clinical and radiological examination, other lesions such as radicular cyst, dentigerous cyst, and ameloblastoma might be considered as the other differential diagnoses (7), but histologically GOC shows certain characteristic features. These features include nonkeratinized stratified squamous lining epithelium, focal thickenings (plaques) within the lining, eosinophilic cuboidal or columnar cells that may be ciliated, mucous cells and mucin pools, and interepithelial gland-like structures (3,4,8,9).

Treatment of GOC includes curettage and enucleation, although some authors believe marginal resection due to a tendency of recurrence after curettage and enucleation (5). In this report, we present a case of GOC associated with impacted tooth. The provisional diagnosis of dentigerous cyst was made, whereas histopathologic examination diagnosed GOC.

 

Case Report

A 24-year old man was referred to Mashhad Faculty of Dentistry, Mashhad, Iran on November 17, 2009 for the evaluation and management of an asymptomatic, firm swelling of the left mandible.

Intraoral examination showed a swelling in the third molar region. A panoramic radiograph demonstrated a well-defined radiolucency surrounding the left mandibular impacted third molar (Fig. 1).

The patient was prepared for the surgical removal of the impacted tooth and the enucleation of the lesion with a provisional diagnosis of dentigerous cyst.

Grossly, the specimen consisted of a brownish-cream cystic mass which was elastic and measured 30Í10Í2 mm.

Histologic examination of the biopsy demonstrated a cystic lesion lined by nonkeratinized stratified squamous epithelium that exhibited the superficial layer of ciliated columnar cells in parts (Fig. 2). Papillary projections into the cyst's lumen and focal epithelial thickenings (plaques) were also observed (Fig 3). Mucous cells with interepithelial mucous pools and gland-like structures were identified throughout the lining epithelium (Figs. 4 and 5). The patient called for three months later and it was observed that the lesion had been healed altogether.

 

 

 

 

Figure 1. A panoramic radiograph shows a well-defined radiolucency, surrounding the left mandibular impacted third molar (arrows)

 

                              

 

Figure 2. Cuboidal eosinophilic cells or "hob-nail" cells and superfacial layer of the cyst with ciliated columnar cells (H&E Original magnification: ×400)

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AA A

 

BA A

 
            

Figure 3. Focal epithelial thickenings (plaque) (A), crypt formation and papillary projections are seen in this section (B) (H&E, Original magnification: ×100)

 

 

 

 

A

A

 

 

A

A

 

 
      

 

B

A

 

 
Figure 4. Mucous cells and interepithelial mucous pools (H&E, Original magnification A: ×400 B:×40)

 

 

 

 

Figure 5. Gland-like structures throughout the lining epithelium (H&E, Original magnification: ×400)

 

 


Discussion

GOC may have a wide clinicopathologic spectrum ranging from a benign small pathology to a destructive neoplasm such as central muoepidermoid carcinoma. Clinically, the most common site of occurrence is mandible (85%), especially in its anterior region (5).

GOC does not display specific or pathognomonic radiographical features. It may be present as a multilocular or unilocular radiolucency with well-defined border, or as perifollicular radiolucency, simulating a hyperplastic follicle or dentigerous cyst (8). Therefore, regarding clinical radiological examination, the diagnosis of other lesions such as radicular cyst, odontogenic keratocyst (OKC), dentigerous cyst and ameloblastoma is made. Only the histopathological examination allows for certain diagnosis of the cyst (7).

In the present case, the clinical diagnosis of dentigerous cyst was made; whereas, histopathologic examination diagnosed a GOC.

Qin et al. (10) reported five maxillary cases contained unerupted teeth. Shimoyama and Horie (11) also reported a cyst in which clinical diagnosis was dentigerous cyst, but histopathological features of were consistent with glandular odontogenic cyst.

According to previous studies GOC is seldom related to unerupted teeth. Histopathological features of the present case satisfied major and minor Kalpan et al. (12) diagnostic criteria for GOC including squamous epithelial lining, flat interface, variation in thickness of the lining with or without epithelial spheres or whorls, no palisade, cuboidal eosinophilic cells or "hob-nail" cells, mucous goblet cells with interepithelial mucous pools with or without crypts lined by mucous-producing cells and interepithelial glandular microcysts or duct like structures as major criteria and papillary projections, ciliated cells, and clear or vacuolated cells in basal or spinous layer as minor criteria. These histological features were also described by other authors (3,4,6).

In GOC, mucous cells are remarkably abundant and the papillary fronds or projections are most exceptional (12). Low grade mucoepidermoid carcinoma (LGMEC) shares a number of histopathological similarities with GOC. In fact, it has been suggested that many cases formerly diagnosed as central MEC can be the examples of GOC, and also some LGMECs would have originated from GOCs (13-15).

Some authors suggested that the distinguishing feature in GOC is the typical thin epithelial lining without any solid epithelial proliferation as seen in MEC. Moreover, swirling spherical (epithelial plaque) that are often seen in GOC are not observed in MEC (16).

In cases where diagnosis of GOC or LGME cannot be made based on the morphological features alone, especially in small incisional biopsy samples, mammary serine protease inhibitor (maspin) immunolocalization can be used to distinguish these two lesions. The high levels of aggregates maspin in the epithelial-mucous cells (in both cytoplasm and nuclei) in LGMEC may serve as a tool to differentiate it from GOC (13).

Pires et al. (15) have demonstrated CK expression in GOC and central MEC. They found differences in CK18 and 19. CK18 is expressed by all MECs, but it is only expressed by 30% of the GOCs, whereas CK19 is expressed by all GOCs and only 50% of the central MECs. They suggested that GOC and central MEC are distinct entities that expression CKs 18 and 19 can be useful adjunctive tools in differentiating these two lesions.

Immunohistochemical studies using cytokeratin 7, 13, 14, and 19 and their positivity strongly support the odontogenic nature (17).

The detection of osteodentin and negative reaction for EMA in the glandular structure show that these features are not glandular origin and support the concept of odontogenic differentiation in GOC (1).

The immunohistochemical expression of the apoptosis- inhibiting protein bcl-2, the cell cycle-related antigen ki-67 and the P53 which is involved both in cell cycle and apoptosis regulation was compared between GOC and dentigerous cyst by I. Tosis et al. (2). The percentage of Ki-67 and P53- positive cells was lower in GOC. They suggested that the biological behavior of GOCs may be associated with deregulation of cell death in the lining epithelium, indicated by increased expression of bcl2, while cell proliferation and P53 do not play a significant role.

The aggressive biologic behavior of the GOC and it's propensity for recurrence might be associated with, cell kinetics in the lining epithelium, as has been demonstrated in OKC (17). It has been also demonstrated that the rate of recurrence increases with the radiographic complexity of the cyst (11). Conservative treatment method is another factor which is responsible for recurrence rate (1). The treatment of choice for GOC has ranged from conservative approach (enucleation, marsupialization, curettage with or without peripheral ostectomy) to marginal resection and segmental resection. Some authors suggest more aggressive removal and long follow-up is necessary (1,5,18).

In conclusion, clinical and radiographic examination of the lesion may result in the diagnosis of other lesions such as radicular cyst, dentigerous cyst and ameloblastoma may be made, but histologically GOC shows certain characteristic features.

 

References

  1. Krishnamurthy AJ, Sherlin H, Ramalingam K, et al. Glandular odontogenic cyst: report of two cases and review of literature. Head Neck Pathol 2009;3:153-8.
  2. Tosis I, Kakarantza K, Angelopoulou E, Kapronas N. Immunohistichemical study of bcl-2 protein, Ki-67 antigen and P53 protein in epithelium of glandular odontogenic cysts and dentigerous cyst. J Oral Pathol Med 2000;29:139-44.
  3. Padyachee A, Van Wyk CW. Two cystic lesions with features of both botryoid odontogenic cyst and the central mucoepidermoid tumor: sialo odontogenic cyst? J Oral Pathol 1987;16:
    499-504.
  4. Gardner DG, Kessler HP, Morency R, Scharner DL. The glandular odontogenic cyst: An apparent entity. J Oral Pathol 1988;17:356-66.
  5. Kasaboglu O, Basal Z, Usubutun A. Glandular odontogenic cyst presenting as a dentigerous cyst: a cases report. J Oral Maxillofacial Surg 2006;64:731-3.
  6. Nair RG, Varghese IV, Shameena PM, Sudha S. Glandular odontogenic cyst: report of a case and review of literature. J Oral Maxillofac Pathol 2006;10:20-3.
  7. Luczak K, Nowak R, Rzeszutko M. Glandular odontogenic cyst of the mandible associated with impacted tooth – report of a case and review of literature. Dent Med Prob 2007;44:403-6.
  8. Sittitavornwong S, Koebler RJ, Said-Al-Naief N. Glandular odontogenic cyst of the anterior maxilla: case report and review of the literature. J Oral Maxillofac Surg 2006;64:740-5.
  9. 9.         Salehinejad J, Saghafi SH, Zare-Mahmoodabadi R, Ghazi N, Kermani H. Glandular odontogenic cyst of the posterior maxilla. Arch Iranian Med 2011;14:416-8.
  10. Qin NX, Li JR, Cben XM, Long X. The glandular odontogenic cyst: clinicopathologic features and treatment of 14 cases. Oral Maxillofac Surg 2005;83:694-9.
  11. Shimoyama T, Horie N. Glandular odontogenic cyst with hyaline bodies: An unusual dentigerous presentation. J Oral Pathol Med1996;25:401-4.
  12. Kalpan I, Anavi Y, Manor R, Sulkes J, Calderson S. The use of molecular markers as an aid in the diagnosis of glandular odontogenic cyst. Oral Oncol 2005;41:895-902.
  13. Vered M, Allon I, Buchner A, Dayan D. Is maspin immunolocalization a tool to differentiate central low-grade mucoepidermoid carcinoma from glandular odontogenic cyst. Acta Histochemica 2010;112:161-8.
  14. Foss DR, Fielding GC. Glandular odontogenic cyst. Head Nek Pathol 2007;1:102-3.
  15. Pires FR, Chen SY, Dacruz Perez DE, Almedia OP, Kowalski LP. Cytokeratin expression in central mucoepidermoid carcinoma and glandular odontogenic cyst. Oral Oncol 2007;40:545-51.
  16. Prabhu S, Rekha K, Kumar G. Glandular odontogenic cyst mimicking central mucoepidermoid carcinoma. J Oral Maxillofac Pathol 2010;14:12-5.
  17. De Soussa SO, Cabexas NT, De Olivera PT, De Araujo VC. Glandular odontogenic cyst report of a case with cytokeratin expression. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;83:
    478-83.
  18. Tabor K, Kakarantza-Angelopoulou E, Kapronas N. The course of long-standing glandular odontogenic cyst: marginal resection with particulated bone graft, platelet-rich plasma and additional vertical alveolar distraction. J Oral Maxillofac Surg 2006;64:1121-8.

 

 

 

Corresponding Author:

Narges Ghazi

Faculty of Dentistry               

Vakilabad Blvd, Mashhad, Iran

P.O. Box: 91735-984

Tel: +98-511-8829501

Fax: +98-511-8829500

E-mail: nargess_ghazi@yahoo.com & ghazin@mums.ac.ir

  1. Krishnamurthy AJ, Sherlin H, Ramalingam K, et al. Glandular odontogenic cyst: report of two cases and review of literature. Head Neck Pathol 2009;3:153-8.
  2. Tosis I, Kakarantza K, Angelopoulou E, Kapronas N. Immunohistichemical study of bcl-2 protein, Ki-67 antigen and P53 protein in epithelium of glandular odontogenic cysts and dentigerous cyst. J Oral Pathol Med 2000;29:139-44.
  3. Padyachee A, Van Wyk CW. Two cystic lesions with features of both botryoid odontogenic cyst and the central mucoepidermoid tumor: sialo odontogenic cyst? J Oral Pathol 1987;16:
    499-504.
  4. Gardner DG, Kessler HP, Morency R, Scharner DL. The glandular odontogenic cyst: An apparent entity. J Oral Pathol 1988;17:356-66.
  5. Kasaboglu O, Basal Z, Usubutun A. Glandular odontogenic cyst presenting as a dentigerous cyst: a cases report. J Oral Maxillofacial Surg 2006;64:731-3.
  6. Nair RG, Varghese IV, Shameena PM, Sudha S. Glandular odontogenic cyst: report of a case and review of literature. J Oral Maxillofac Pathol 2006;10:20-3.
  7. Luczak K, Nowak R, Rzeszutko M. Glandular odontogenic cyst of the mandible associated with impacted tooth – report of a case and review of literature. Dent Med Prob 2007;44:403-6.
  8. Sittitavornwong S, Koebler RJ, Said-Al-Naief N. Glandular odontogenic cyst of the anterior maxilla: case report and review of the literature. J Oral Maxillofac Surg 2006;64:740-5.
  9. 9.         Salehinejad J, Saghafi SH, Zare-Mahmoodabadi R, Ghazi N, Kermani H. Glandular odontogenic cyst of the posterior maxilla. Arch Iranian Med 2011;14:416-8.
  10. Qin NX, Li JR, Cben XM, Long X. The glandular odontogenic cyst: clinicopathologic features and treatment of 14 cases. Oral Maxillofac Surg 2005;83:694-9.
  11. Shimoyama T, Horie N. Glandular odontogenic cyst with hyaline bodies: An unusual dentigerous presentation. J Oral Pathol Med1996;25:401-4.
  12. Kalpan I, Anavi Y, Manor R, Sulkes J, Calderson S. The use of molecular markers as an aid in the diagnosis of glandular odontogenic cyst. Oral Oncol 2005;41:895-902.
  13. Vered M, Allon I, Buchner A, Dayan D. Is maspin immunolocalization a tool to differentiate central low-grade mucoepidermoid carcinoma from glandular odontogenic cyst. Acta Histochemica 2010;112:161-8.
  14. Foss DR, Fielding GC. Glandular odontogenic cyst. Head Nek Pathol 2007;1:102-3.
  15. Pires FR, Chen SY, Dacruz Perez DE, Almedia OP, Kowalski LP. Cytokeratin expression in central mucoepidermoid carcinoma and glandular odontogenic cyst. Oral Oncol 2007;40:545-51.
  16. Prabhu S, Rekha K, Kumar G. Glandular odontogenic cyst mimicking central mucoepidermoid carcinoma. J Oral Maxillofac Pathol 2010;14:12-5.
  17. De Soussa SO, Cabexas NT, De Olivera PT, De Araujo VC. Glandular odontogenic cyst report of a case with cytokeratin expression. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;83:
    478-83.
  18. Tabor K, Kakarantza-Angelopoulou E, Kapronas N. The course of long-standing glandular odontogenic cyst: marginal resection with particulated bone graft, platelet-rich plasma and additional vertical alveolar distraction. J Oral Maxillofac Surg 2006;64:1121-8.