TY - JOUR ID - 88 TI - Comparative Assessment of Nuclear and Nucleolar Cytochemical Parameters of Oral Epithelial Cells in Smokers and Non-Smokers by Methyl Green-Pyronin Staining JO - Journal of Dental Materials and Techniques JA - JDMT LA - en SN - 2322-4150 AU - Salehinejad, Jahanshah AU - Mahdavi-Shahri, Nasser AU - Mohtasham, Nooshin AU - Ejtehadi, Hamid AU - Adhami, Shahrzad AU - Pazouki, Mahdi AD - Dental Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran AD - Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Kerman University of Medical Sciences, Kerman, Iran Y1 - 2012 PY - 2012 VL - 1 IS - 1 SP - 19 EP - 23 KW - Cytochemistry KW - methyl green-pyronine KW - nucleolus KW - oral mucosa KW - Smoking DO - 10.22038/jdmt.2013.88 N2 - Introduction: A strong relationship exists between cigarette smoking and the development of oral squamous cell carcinoma. Smoking can significantly increase cellular proliferation. Nevertheless, there is little reference in literature to the cytological assessment of oral mucosa in this respect. Methods: Changes in nuclear and neucleolar cytomorphometric parameters such as diameter, surface, number and color intensity, in cytologic smears which were collected from normal buccal mucosa of 30 cigarette smokers and 30 non smokers, using methyl green-pyronin staining were studied. Results: Our findings attested to smoking as significant inductive factor in cytochemistry as well as morphologic changes. Conclusion: This technique is a valuable tool UR - https://jdmt.mums.ac.ir/article_88.html L1 - https://jdmt.mums.ac.ir/article_88_ef9ef20db818c77aac25195c7224e77f.pdf ER -